Microbiological Culture
- Introduction
Microbiological culture, or microbial culture, is a well-established research tool in molecular biology for the culture of bacteria and yeast organisms.
The use of solid culture media has been of fundamental importance to microbiological research since the late nineteenth century. Desirable qualities of a solidifying agent for media include solidity over the temperature range of bacterial growth, resistance to digestion by bacteria, lack of syneresis, transparency, and the ability to form a reversible colloid. The medium must be firm enough to allow the carrying out of common techniques such as streaking out cultures, plating, and replication.
In addition, it is helpful if the gelation agent is relatively inexpensive and easily obtained.
- Existing Products
We offer a wide array of solidifying agents to help you achieve your desired results.
- Bacteriological Agar is widely used for solid culture media in microbiology. Solidified media using agar as a solidifying agent are indispensable in microbiology. For solid cultures of mesophilic microorganisms, agar is an ideal solidifying agent and has been used essentially unchanged since it was first introduced in the late 19th century. In microbiology or solid culture media agar is used at a concentration of 1 to 2%. Of course agar has been known for more than a hundred years as culture media for the separation of bacterial cultures in bacteriology.
- Agarose
- Gellan gum
- Carrageenan: The potassium salt of the sulfated polysaccharide, carrageenan, is an adequate substitute for agar in most common genetic manipulations of Escherichia coli.
- Applications
Clinical applications, auxotrophic studies, bacterial and yeast transformation studies, and bacterial molecular genetics applications.
- Features & Advantages
Desirable qualities of a solidifying agent for media include:
Solidity over the temperature range of bacterial growth,
Resistance to digestion by bacteria,
Lack of syneresis, transparency,
and the ability to form a reversible colloid.
The medium must be firm enough to allow the carrying out of common techniques such as streaking out cultures, plating, and replication.
In addition, it is helpful if the gelation agent is relatively inexpensive and easily obtained.
- Guidelines for use
Agar is typically used in a final concentration of 1 - 2% for solidifying culture media. Smaller quantities (0.05 - 0.5%) are used in media for motility studies (0.5% w/v), growth of anaerobes (0.1%), and microaerophiles.
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